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abstractpubmed· Abstract 2018· item PMID:30466559

Kidney organoids-a new tool for kidney therapeutic development. Kidney organoids, derived from human pluripotent stem cells, have the potential to greatly facilitate drug development. Boreström et al. have used CRISPR/Cas9 to create kidney fluorescent lineage markers for SIX2 and NPHS1 to monitor the differentiation process to tubular and glomerular structures and optimize maturity. The convergence of "personalized" kidney organoids with genome editing and single-cell sequencing technology hold great promise to result in better insight to disease, better human cell disease models, more predictive toxicology, and potentially "clinical trials in a dish."