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Potentially Pathogenic Leptospira Species Isolated from a Waterfall in Thailand. We collected water and soil samples from a waterfall in Thailand to investigate the presence of potentially pathogenic Leptospira. Isolation of Leptospira from all the 17 environmental samples was successful. Based on 16S rRNA gene sequence analysis, a diverse group of Leptospira species was recovered from waterfall samples including 2 pathogenic species (Leptospira alstonii [5/17, 29%] and Leptospira kmetyi [1/17, 6%]); 1 intermediate species (Leptospira wolffii [9/17, 53%]); and 2 non-pathogenic species (Leptospira meyeri [1/17, 6%] and Leptospira idonii [1/17, 6%]). The high prevalence of pathogenic and intermediate Leptospira indicates that a waterfall may serve as a natural reservoir of possible pathogens of leptospirosis.