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abstractpubmed· Abstract 2015· item PMID:25531672

Use of Treponema pallidum PCR in testing of ulcers for diagnosis of primary syphilis. Treponema pallidum PCR (Tp-PCR) has been noted as a valid method for diagnosing syphilis. We compared Tp-PCR to a combination of darkfield microscopy (DFM), the reference method, and serologic testing in a cohort of 273 patients from France and Switzerland and found the diagnostic accuracy of Tp-PCR was higher than that for DFM.