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abstractpubmed· Abstract 2019· item PMID:31625866
Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada. Legionellosis was diagnosed in an immunocompromised 3-year-old girl in Canada. We traced the source of the bacterium through co-culture with an ameba collected from a hot tub in her home. We identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm the environmental and clinical isolates were of common origin.