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Rotavirus infection is a leading cause of gastroenteritis in children <5 years of age worldwide; children with severe dehydration and electrolyte imbalance require hospitalization (1). In 2007 and 2008, the annual economic cost for hospitalization of rotavirus case-patients in Israel was US $4,578,489 in direct costs to the health system plus an additional household cost of $3,101,955 (2). Poor hygienic conditions and lack of appropriate medical facilities in developing countries result in high infant mortality rates (1,3–5). The genes encoding the outer capsid viral proteins VP7 and VP4 form the basis of classification of group A rotaviruses into G and P genotypes, respectively (6,7). Two live oral vaccines, monovalent Rotarix vaccine (GlaxoSmithKline, Research Triangle Park, NC, USA) and pentavalent Rotatek vaccine (Merck, Rahway, NJ, USA) effectively reduced hospitalizations for subsequent infections with G1P[8], G2P[4], G3P[8], and G4P[8] rotaviruses by >85% (8,9). Rotavirus vaccines that are scheduled for inclusion in Israel’s national vaccination program after 2010 may selectively change relative distributions of naturally cocirculating rotaviruses of different G and P genotypes. According to analysis of voluntary immunizations before universal vaccination, the effectiveness of rotavirus vaccine in preventing rotavirus gastroenteritis–associated hospitalizations in Israel is already evident (10).

hange relative distributions of naturally cocirculating rotaviruses of different G and P genotypes. According to analysis of voluntary immunizations before universal vaccination, the effectiveness of rotavirus vaccine in preventing rotavirus gastroenteritis–associated hospitalizations in Israel is already evident (10). Simple to use, inexpensive, diagnostic rotavirus rapid test strips (dipsticks) identify group A rotavirus–positive stools in <30 minutes by lateral flow immunochromatography without indicating the genotype. Dipsticks are dipped into saline stool suspensions. If rotavirus is present, a colored band appears when indicator-linked rotavirus antibodies bind to the virus, and this complex is trapped on a band of membrane-bound rotavirus antibody on the dipstick. With their growing use at point-of-care facilities and in local hospital laboratories, fewer samples are available for genotyping by centralized reference laboratories. Obtaining geographically representative stool samples and expanding surveillance to regions lacking adequate laboratory facilities is becoming increasingly difficult. The ease with which dipsticks can be stored at local facilities and transported to central reference laboratories can help overcome these difficulties. We hypothesized that rotavirus trapped on rotavirus-specific antibody bands, equivalent to affinity concentrated rotavirus, might yield genotyping-quality RNA from dipsticks that are easily archived locally and transportable to centralized laboratories. In this study, our main objective was to determine the feasibility of recovering molecular epidemiologic data from rotavirus immobilized on air-dried diagnostic rotavirus dipsticks to compensate for the decreasing number of fecal samples reaching central reference laboratories.

ransportable to centralized laboratories. In this study, our main objective was to determine the feasibility of recovering molecular epidemiologic data from rotavirus immobilized on air-dried diagnostic rotavirus dipsticks to compensate for the decreasing number of fecal samples reaching central reference laboratories. Methods Stool samples from 311 children hospitalized with acute gastroenteritis at Sheba Medical Center, Tel Hashomer, Israel, during May 15, 2005–May 15, 2006, were suspended in saline. Rotavirus dipsticks (Hy Laboratories LTD, Rehovot, Israel) were used to identify rotavirus-positive stool samples. (For comparison with rotavirus ELISA with Dako reagents [Dako Diagnostics LTD, Glostrup, Denmark], diagnostic dipsticks from Hy-Laboratories and Novamed [Novamed, Jerusalem, Israel] had sensitivities of 98.5% ± 3% and 92.3%, and specificities of 92.5% ± 1% and 93.5%, respectively [L.M. Shulman et al., unpub data].) The section of the dipstick containing the rotavirus-specific bands from 14 rotavirus-positive dipsticks that had been stored at room temperature for at least 1 week were excised and placed into 120 µL saline.

5% ± 3% and 92.3%, and specificities of 92.5% ± 1% and 93.5%, respectively [L.M. Shulman et al., unpub data].) The section of the dipstick containing the rotavirus-specific bands from 14 rotavirus-positive dipsticks that had been stored at room temperature for at least 1 week were excised and placed into 120 µL saline. Rotaviral RNA was extracted from these rotavirus-specific bands and from the corresponding saline stool suspensions by using QIAmp Viral RNA Mini kits (QIAGEN, Valencia, CA, USA). RNA amplified from rotavirus-specific bands from dipsticks archived at room temperature 5 years earlier was similarly extracted. RNA was amplified by using generic primers for reverse transcription–PCR (RT-PCR) (QIAGEN OneStep RT-PCR kits, QIAGEN) followed by heminested PCR (AmpliTaq Gold, Applied Biosystems, Foster City, CA, USA) with genotype-specific primers to identify G and P genotypes as described by Gouvea et al. (11) and Gunasena et al. (12), respectively. RT-PCR products were gel purified (QIAQuick Gel Extraction Kit, QIAGEN) or purified directly (HiPure PCR Product Purification Kit; Roche Applied Science, Indianapolis, IN, USA) from the PCR mix and sequenced by using an ABI PRISM Dye Deoxy Terminator cycle sequencing kit (Applied Biosystems) and the genotype-specific primer and common primer from the heminested PCR reaction described. Reaction mixtures were analyzed on Applied Biosystems model 373 DNA automatic sequencing system to confirm the RT-PCR genotyping results and for phylogenetic analysis.

eoxy Terminator cycle sequencing kit (Applied Biosystems) and the genotype-specific primer and common primer from the heminested PCR reaction described. Reaction mixtures were analyzed on Applied Biosystems model 373 DNA automatic sequencing system to confirm the RT-PCR genotyping results and for phylogenetic analysis. Sequences of the same genotype were truncated to the longest common length by using the Sequencher program (Genecodes, Ann Arbor, Michigan, USA). Nearest-neighbor phylogenetic trees were constructed from these sequences after the data were bootstrapped 1,000 times with Clustal X (13). The tree was analyzed by using NJplot (14). Sequences have been deposited in European Molecular Biology Laboratory/GenBank/DNA Data Bank of Japan under accession nos. FN298858–FN298875, FN582119–FN582124, and HQ174462–HQ174463. (Isolate names are linked to their specific accession numbers in Table A1). This study was approved by the institutional review board of Chaim Sheba Medical Center (SMC-7606-09). Experiments were not performed on humans. All personal identification was removed from the remnants of fecal samples sent to the National Center for Viral Gastroenteritis for rotavirus analysis. The viral RNA used for this study was obtained from these anonymous samples.

ard of Chaim Sheba Medical Center (SMC-7606-09). Experiments were not performed on humans. All personal identification was removed from the remnants of fecal samples sent to the National Center for Viral Gastroenteritis for rotavirus analysis. The viral RNA used for this study was obtained from these anonymous samples. Results To assess the suitability of RNA extracted from dipsticks for determining the VP7 genotype, we first determined the VP7 G genotype of rotavirus in rotavirus-positive clinical samples from children hospitalized during May 15, 2005–May 15, 2006. Of 311 stool samples, 61 were determined to be rotavirus positive by using diagnostic rotavirus dipsticks. The G genotype and P genotype of RNA in 54 of the 61 samples was determined by the size of the heminested PCR amplification product as described. Mixed infections, e.g., >1 G (6 samples) and P genotypes (2 samples) in the same fecal suspension, were found in 8 (15%) of the 54 positive samples. Taking into account mixed infections, we found G1, G2, G3, and G9 G genotypes in 79%, 5%, 2%, and 15%, respectively, of the 62 rotaviruses identified in the 54 clinical samples. The frequencies of associated G and P genotypes for isolates from the 2005–2006 rotavirus season were 3% G1P[4], 76% G1P[8], 5% G2P[4], 2% G3P[8], 3% G9P[4], and 11% G9P[8].

tions, we found G1, G2, G3, and G9 G genotypes in 79%, 5%, 2%, and 15%, respectively, of the 62 rotaviruses identified in the 54 clinical samples. The frequencies of associated G and P genotypes for isolates from the 2005–2006 rotavirus season were 3% G1P[4], 76% G1P[8], 5% G2P[4], 2% G3P[8], 3% G9P[4], and 11% G9P[8]. We chose 18 rotavirus-positive clinical samples with which to study the feasibility of recovering molecular information from dipsticks. Thirteen were from children infected with 1 G genotype of rotavirus and 5 from children simultaneously infected with rotaviruses belonging to 2 different G genotypes. Of the 13 infections by a single G genotype, 8 were G1, 3 were G2, and 1 each were G3 and G9. All 5 mixed infections, RoV-24_ISR05, RoV_41_ISR05, RoV_56_ISR06, RoV_57_ISR06, and RoV_60_ISR06, were simultaneous infections with G1 and G9 genotypes. Mixed infections were identified by specific product size on gels and confirmed by partial sequencing of the G1 product for all 4 patients whose G9 sequences appear in the Figure.

infections, RoV-24_ISR05, RoV_41_ISR05, RoV_56_ISR06, RoV_57_ISR06, and RoV_60_ISR06, were simultaneous infections with G1 and G9 genotypes. Mixed infections were identified by specific product size on gels and confirmed by partial sequencing of the G1 product for all 4 patients whose G9 sequences appear in the Figure. Figure Neighbor-joining phylogenetic trees for viral protein (VP) 7 G1, G2, G3, and G9 genotypes of hospitalized children in Israel, including sequences recovered from archived rotavirus dipsticks. Representative isolates for lineages and sublineages of VP7 genotypes G1 (A), G2 (B), G3 (C), and G9 (D) were chosen from Phan et al. (15) Page and Steele (16), Wang et al. (17), and Martinez-Laso et al. (18), respectively, and the sequences were downloaded from the European Molecular Biology Laboratory/GenBank/DNA Data Bank of Japan. These sequences were aligned with Israeli sequences by using the Sequencher program (Genecodes, Ann Arbor, MI, USA) and truncated to the longest segment common to all sequences in the alignment; 515 nt for G1, 547 nt for G2, 274 nt for G3, and 207 nt for G9. Each of the 4 phylogenetic trees was prepared by using ClustalX (13) for data bootstrapped 1,000× and was analyzed with NJplot (14). Whole numbers indicate bootstrap values for branches; fractional numbers indicate genetic distances. The genotype, lineage, and, where relevant, sublineage of each isolate appears in brackets after the name of the isolate: for example, KY3303[G2.2a] is VP7 genotype G 2, lineage 2, sublineage a for isolate KY3303. A letter at the end of the name of the Israeli sequences indicates the source of the RNA (D for dipstick or S for fecal suspension). D&S appears when the sequences were identical. The GenBank accession numbers for all sequences in this figure appear in Table A1. Scale bars indicate percent of nucleotide substitutions per site.

at the end of the name of the Israeli sequences indicates the source of the RNA (D for dipstick or S for fecal suspension). D&S appears when the sequences were identical. The GenBank accession numbers for all sequences in this figure appear in Table A1. Scale bars indicate percent of nucleotide substitutions per site. RNA was extracted from rotavirus-positive bands of dipsticks from the 18 clinical samples. The dipsticks had been stored for >1 week at room temperature. The quality of the RNA from all 18 extractions was sufficient to enable determination of VP7 G genotypes by heminested RT-PCR. The genotypes obtained from the RNA extracted from dipsticks were identical to those from RNA extracted from corresponding saline suspensions for 14 of the 18 rotavirus-positive stool samples, including 3 samples from children with dual infections. In 2 mixed G1–G9 infections, G9, but not G1 RNA, was recovered, and G1 RNA was recovered from dipstick RNA in addition to G9 from a G9-infected child. A fourth dipstick from a G3 sample yielded a G3 and an equivocal G1 that did not appear in subsequent replicate heminested amplifications. In other words, 21 (92%) of 23 genotypes identified from RNA extracted from 18 fecal suspensions were also identified from RNA extracted from dipsticks, including 8 of 10 genotypes from 5 samples simultaneously infected with rotaviruses of 2 different genotypes.

appear in subsequent replicate heminested amplifications. In other words, 21 (92%) of 23 genotypes identified from RNA extracted from 18 fecal suspensions were also identified from RNA extracted from dipsticks, including 8 of 10 genotypes from 5 samples simultaneously infected with rotaviruses of 2 different genotypes. Rotaviral VP7 RNA suitable for genotyping was also recovered from all 5 air-dried rotavirus-positive dipsticks that had been stored at room temperature for 5 years. The G genotypes of the rotavirus in the fecal suspensions from the 5 archived dipsticks from 2002 were unknown, and the suspensions were no longer available for comparison. One of the dipsticks was manufactured by Hy Laboratories; the remaining 4 were manufactured by Novamed. Initial amplification with generic G-type primers yielded generic G genotype amplification products of expected size (≈1,062 nt) upon gel electrophoresis. Three of the dipsticks yielded G3 RNA; the other 2 yielded G1 RNA. As proof of concept that P genotypes could be determined in addition to G genotypes, the P genotypes of RNA recovered from 2 of the 5-year-old rotavirus dipsticks with G3 RNA were also determined by direct sequencing of the RT-PCR products by using the generic P-type primers. Both were P[8] by sequence analysis (Table A1).

roof of concept that P genotypes could be determined in addition to G genotypes, the P genotypes of RNA recovered from 2 of the 5-year-old rotavirus dipsticks with G3 RNA were also determined by direct sequencing of the RT-PCR products by using the generic P-type primers. Both were P[8] by sequence analysis (Table A1). The G genotypes of 3 G1s, 2 G2s, 1 G3, and 1 G9 RNA extracted from dipsticks were confirmed by sequencing their heminested amplification products. Moreover, the sequences of the RNA extracted from each of the 7 dipsticks were identical to the sequence of the RNA extracted directly from the corresponding stool suspension. The lineages and sublineages of these and other isolates from the 2005–06 season were inferred from phylogenetic comparisons to equivalent segments of the 11 lineages of G1, the 2 lineages of G2, the 4 lineages of G3, and the 6 lineages of G9 described by Phan et al. (15), Page and Steele (16), Wang et al. (17), and Martinez-Laso et al. (18), respectively. The G3 phylogenetic tree also includes 3 sequences obtained from the 5-year-old dipsticks and 4 G3 isolates from among those with highest homology to G3 isolates from Israel identified in BLAST searches (http://blast.ncbi.nlm.nih.gov/Blast.cgi) (19). Specifically, all VP7 sequences for a given G genotype were aligned and truncated to the longest common segment. Four separate phylogenetic trees (Figure) were constructed because of the size difference between G genotype–specific heminested amplification products. The lineage or sublineage of each isolate from Israel (Table A) was inferred from the relative difference of each sequence compared with equivalent segments of previously determined lineages. For each genotype, a new lineage or sublineage was suggested when the sequence from Israel failed to closely group with the equivalent segment of >1 reference strains and the nucleotide differences between the strain from Israel and the reference strains were similar or greater than the differences among reference lineages and sublineages. The existence of such new lineages and sublineages would need to be confirmed with longer full-length open reading frame sequences. Finally, sequences from RNA recovered from dipsticks and/or from saline suspensions were used in BLAST searches to identify the most similar contemporary sequences.

neages and sublineages. The existence of such new lineages and sublineages would need to be confirmed with longer full-length open reading frame sequences. Finally, sequences from RNA recovered from dipsticks and/or from saline suspensions were used in BLAST searches to identify the most similar contemporary sequences. G1 isolates were most similar to isolates from Europe, the Far East, and South America; G2 isolates to isolates from the Far East; and G3 isolates to isolates from Europe and the Far East (Figure). G9 isolates were most similar to isolates from Africa, Europe, and the Far East.

neages and sublineages. The existence of such new lineages and sublineages would need to be confirmed with longer full-length open reading frame sequences. Finally, sequences from RNA recovered from dipsticks and/or from saline suspensions were used in BLAST searches to identify the most similar contemporary sequences. G1 isolates were most similar to isolates from Europe, the Far East, and South America; G2 isolates to isolates from the Far East; and G3 isolates to isolates from Europe and the Far East (Figure). G9 isolates were most similar to isolates from Africa, Europe, and the Far East. Discussion The G1P[8] genotype predominated in central Israel during the 2005–06 rotavirus season as it did throughout Israel during 1991–1994 (20) and in northern Israel during 2007–2009 (2,10). However, the relative distributions of other genotypes differed. For example, G4P[8], absent during 2005–2008, was present in 32.3% of samples a decade earlier and reappeared in 2008–09 (2). Conversely, G9 genotypes, absent a decade earlier, were present in 15.7% of the 2005–06 isolates and 9.3% of the 2006–07 isolates. This substantial prevalence of G9 mirrors the global emergence of G9 among hospitalized children in the mid-1990s (21,22). In addition, double infections indicated by >1 G or P genotype rose from <2% during 1991–1994 (20) to 16.4% for the isolates in this study and 27% during 2007–08 (2). As indicated by Muhsen et al (2), evidence is good for rotavirus reassortants emerging in vivo from appropriate double infections. Phylogenetic analysis in the present study suggested that some G2 and G9 rotaviruses from Israel belonged to new sublineages or lineages, respectively.

his study and 27% during 2007–08 (2). As indicated by Muhsen et al (2), evidence is good for rotavirus reassortants emerging in vivo from appropriate double infections. Phylogenetic analysis in the present study suggested that some G2 and G9 rotaviruses from Israel belonged to new sublineages or lineages, respectively. In conclusion, air-dried affinity-concentrated virus from rotavirus-positive bands of dipsticks yielded RNA suitable for G genotyping and sequencing, even for dipsticks stored at room temperature for 5 years. Even though P genotyping and sequencing was performed only on RNA isolated from 2 of the 5-year old dipsticks, it is reasonable to assume that P genotyping and sequencing would have been possible for the rest based on the equivalent quality of the RNA extracted from the dipsticks and RNA extracted from stool suspensions. Thus, dipsticks can be used for recovery of molecular epidemiologic data by reference laboratories from diagnostic tests routinely performed in many clinical laboratories and point-of-care facilities. RNA extracted from these archived dipsticks will enable centralized laboratories to easily recover epidemiologically useful data from the samples of other laboratories and from regions lacking adequate laboratory facilities. In addition, centralized laboratories will be able to assess whether the introduction of universal rotavirus vaccination changed the distribution of rotavirus genotypes associated with severe rotavirus-associated acute gastroenteritis.

mples of other laboratories and from regions lacking adequate laboratory facilities. In addition, centralized laboratories will be able to assess whether the introduction of universal rotavirus vaccination changed the distribution of rotavirus genotypes associated with severe rotavirus-associated acute gastroenteritis. Suggested citation for this article: Shulman LM, Silberstein I, Alfandari J, Mendelson E. Genotyping rotavirus RNA from archived rotavirus-positive rapid test strips. Emerg Infect Dis [serial on the Internet]. 2011 Jan [date cited]. http://dx.doi.org/10.3201/eid1701.101132 Acknowledgment We dedicate this article to the memory of J.M. Paswell of the Department of Pediatrics, Chaim Sheba Medical Center, Tel Hashomer, Israel. Portions of this study were started in collaboration with him, and he contributed to its initial planning. GlaxoSmithKline provided J.M. Paswell with the funds to purchase the RT-PCR primers used for the rotavirus genotyping in this study. GlaxoSmithKline had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The Israel Ministry of Health funded all expenses except the primers.

ine provided J.M. Paswell with the funds to purchase the RT-PCR primers used for the rotavirus genotyping in this study. GlaxoSmithKline had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The Israel Ministry of Health funded all expenses except the primers. Table A1 Rotavirus genotypes and reference isolates, Israel Genotype and lineage Sublineage Isolate name* GenBank accession no. Reference G1 genotype 515 nt (equivalent to nt 479–nt 993 of isolate VN-281 (DQ508167) 1 a Dhak8 AY631049 (15) 1 b VN-281 DQ508167 (15) 1 c Mvd9816 AF480293 (15) 1 d 6916/JP EF079067 (15) 1 e CH631 AF183837 (15) 2 a DC03 AF183859 (15) 2 b PA5/03 DQ377596 (15) 2 c JP-7026 EF079065 (15) 2 d PA164 DQ377588 (15) 2 e Russia-407 S83903 (15) 3 b Israel-56 U26376 (15) 4 None K54 U26377 (15) 5 None PA5/90 QD377573 (15) 6 None AU19 AB018697 (15) 7 None Ban-56 U26366 (15) 8 None Egypt-7 U26373 (15) 9 A P19 DQ377593 (15) 10 None SW2021 AF426162 (15) 11 b C60 L24164 (15) 2 c RoV_09_ISR05[G1.2c]D FN582119 New 2 c RoV_09_ISR05[G1.2c]S FN298858 New 1 a RoV_10_ISR05[G1.1a]D FN582120 New 1 a RoV_10_ISR05[G1.1a]S FN582121 New 2 c RoV_14_ISR05[G1.2c]S FN582122 New 1 a RoV_41_ISR05[G1.1a]D FN582123 New 1 a RoV_41_ISR05[G1.1a]S FN298860 New G2 genotype 547 nt (equivalent to nt 455–nt 1,001 of isolate TN1529 (AY261357) 1 None sa514 gr ay261338 (16) 2 a KY3303 AY261350 (16) 2 b SA3958GR AY261344 (16) 2 c TN1529 AY261357 (16) 2 d BF3767 AY261355 (16) 2 e† RoV_39_ISR05[G2.2e]D FN582124 New 2 e† RoV_39_ISR05[G2.2e]S FN298861 New 2 B RoV_51_ISR05[G2.2b]S FN298862 New 2 e† RoV_63_ISR06[G2.2e]D FN298864 New 2 e† RoV_63_ISR06[G2.2e]S FN298863 New G3 genotype 274 nt (equivalent to nt 701–nt 982 of isolate AU-1 (D86271) 1 None CMH222 AY707792 (17) 2 None CHW17 D86276 (17) 2 None CH-55 D86274 (17) 3 1 AU-1 D86271 (17) 3 2 TK28 D86283 (17) 3 3 CMH120/04 DQ923797 (17) 3 4 E887 EU708579 (17) 3 4 CMH272 AY707790 GenBank 3 4 Rus32 EF495127 GenBank 3 4 47–2536Madrid DQ440624 GenBank 3 4 VN-933 EF495123 GenBank 4 None CMP096 (porcine) DQ256502 (17) 3 4 RoV_28_ISR05[G3.3.4]D FN298865 New 3 4 RoV_28_ISR05[G3.3.4]S FN298866 New 3 4 RoV_64_ISR02[G3.3.4]D‡ FN298867 New 3, NA¶ 4, NA RoV_65_ISR02[G3.3.4]D,‡ RoV_65_ISR02[P[8]]D§ FN298868, HQ174462 New 3, NA 4, NA RoV_68_ISR02[G3.3.4]D,‡ RoV_68_ISR02[P[8]]D§ FN298869, HQ174463 New G9 genotype 207 nt (equivalent to nt 77–nt 983 of isolate AU32 (AB045372) 1 None.

4 RoV_28_ISR05[G3.3.4]S FN298866 New 3 4 RoV_64_ISR02[G3.3.4]D‡ FN298867 New 3, NA¶ 4, NA RoV_65_ISR02[G3.3.4]D,‡ RoV_65_ISR02[P[8]]D§ FN298868, HQ174462 New 3, NA 4, NA RoV_68_ISR02[G3.3.4]D,‡ RoV_68_ISR02[P[8]]D§ FN298869, HQ174463 New G9 genotype 207 nt (equivalent to nt 77–nt 983 of isolate AU32 (AB045372) 1 None. AU32 AB045372 (18) 2 None 116E L14072 (18) 3 None INL1 AJ250277 (18) 4 None 97'SZ37 AF260959 (18) 5 None OM67 AJ491179 (18) 6 None SP1542 AB091753 (18) 7† None RoV_24_ISR05[G9.7]S FN298870 New 8† None RoV_56_ISR06[G9.8]D FN298873 New 8† None RoV_56_ISR06[G9.8]S FN298872 New 8† None RoV_57_ISR06[G9.8]S FN298874 New 8† None RoV_60_ISR06[G9.8]S FN298875 New *Isolate names were taken from European Molecular Biology Laboratory/GenBank/DNA Data Bank of Japan. Israeli samples have been named RoV_the isolate number_ISR for Israel and a 2-digit code indicating the year the sample was sent to the laboratory for initial testing. †New lineages or sublineages inferred by phylogenetic comparison to equivalent segments from reference lineages and sublineages, as described in the text. ‡G genotypes determined from RNA isolated from dipsticks stored at room temperature for 5 y. RNA from all other dipsticks was extracted 1–6 wk after the rapid test strips (dipsticks) were used. §P genotypes determined from RNA isolated from dipsticks stored at room temperature for 5 y. The 457-nt sequence is equivalent to nt 222– 678 of a G3P[8] isolate, 95–87 (AB008290). ¶NA, not applicable.

perature for 5 y. RNA from all other dipsticks was extracted 1–6 wk after the rapid test strips (dipsticks) were used. §P genotypes determined from RNA isolated from dipsticks stored at room temperature for 5 y. The 457-nt sequence is equivalent to nt 222– 678 of a G3P[8] isolate, 95–87 (AB008290). ¶NA, not applicable. Dr Shulman is head of the Environmental Virology Laboratory and the National Center for Viral Gastroenteritis in the Central Virology Laboratory, Public Health Services Laboratory, Israel Ministry of Health and a senior lecturer in the Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel Aviv University. His primary research interests include molecular epidemiology of polio and nonpolio enteroviruses and viruses that cause acute gastroenteritis, genomic reorganization in human BK polyomavirus after organ transplantation, and poliovirus environmental surveillance.